Nuclear DNA content of the hybrid plant pathogen Phytophthora andina determined by flow cytometry.
Identifieur interne : 000B66 ( Main/Exploration ); précédent : 000B65; suivant : 000B67Nuclear DNA content of the hybrid plant pathogen Phytophthora andina determined by flow cytometry.
Auteurs : Jianan Wang [États-Unis] ; Jackson W. Presser [États-Unis] ; Erica M. Goss [États-Unis]Source :
- Mycologia [ 0027-5514 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN.
- génétique : Phytophthora.
- isolement et purification : Phytophthora.
- microbiologie : Plantes.
- Cytométrie en flux, Mexique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- genetics : Phytophthora.
- isolation & purification : Phytophthora.
- microbiology : Plants.
- Flow Cytometry, Mexico.
Abstract
Phytophthora andina is a heterothallic plant pathogen of Andean solanaceous hosts and is an interspecific hybrid of P. infestans and an unknown Phytophthora species. The objective of this study was to estimate the nuclear DNA content of isolates in three clonal lineages of P. andina relative to P. infestans Twelve isolates of P. andina and six isolates of P. infestans were measured for nuclear DNA content by propidium iodide-stained flow cytometry. We found that the DNA content of P. andina was similar but slightly smaller, on average, than that of our sample of P. infestans isolates. This is consistent with P. andina being a homoploid hybrid rather than allopolyploid hybrid. Nuclear DNA content was more variable among a smaller sample of P. infestans isolates, including a putative triploid isolate from Mexico, but small differences in nuclear DNA content were also observed among P. andina isolates. Both species appear to be able to tolerate significant variation in genome size.
DOI: 10.3852/15-107
PubMed: 27302049
Affiliations:
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Presser</name><affiliation wicri:level="2"><nlm:affiliation>School of Natural Resources and Environment and Emerging Pathogens Institute, PO Box 116455, University of Florida, Gainesville, Florida 32611.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>School of Natural Resources and Environment and Emerging Pathogens Institute, PO Box 116455, University of Florida, Gainesville</wicri:cityArea></affiliation></author><author><name sortKey="Goss, Erica M" sort="Goss, Erica M" uniqKey="Goss E" first="Erica M" last="Goss">Erica M. 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Goss</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology and Emerging Pathogen Institute, PO Box 110680, University of Florida, Gainesville, Florida 32611 emgoss@ufl.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Emerging Pathogen Institute, PO Box 110680, University of Florida, Gainesville</wicri:regionArea><wicri:noRegion>Gainesville</wicri:noRegion></affiliation></author></analytic><series><title level="j">Mycologia</title><idno type="ISSN">0027-5514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (analysis)</term><term>Flow Cytometry (MeSH)</term><term>Mexico (MeSH)</term><term>Phytophthora (genetics)</term><term>Phytophthora (isolation & purification)</term><term>Plants (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>Cytométrie en flux (MeSH)</term><term>Mexique (MeSH)</term><term>Phytophthora (génétique)</term><term>Phytophthora (isolement et purification)</term><term>Plantes (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Flow Cytometry</term><term>Mexico</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cytométrie en flux</term><term>Mexique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora andina is a heterothallic plant pathogen of Andean solanaceous hosts and is an interspecific hybrid of P. infestans and an unknown Phytophthora species. The objective of this study was to estimate the nuclear DNA content of isolates in three clonal lineages of P. andina relative to P. infestans Twelve isolates of P. andina and six isolates of P. infestans were measured for nuclear DNA content by propidium iodide-stained flow cytometry. We found that the DNA content of P. andina was similar but slightly smaller, on average, than that of our sample of P. infestans isolates. This is consistent with P. andina being a homoploid hybrid rather than allopolyploid hybrid. Nuclear DNA content was more variable among a smaller sample of P. infestans isolates, including a putative triploid isolate from Mexico, but small differences in nuclear DNA content were also observed among P. andina isolates. Both species appear to be able to tolerate significant variation in genome size.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27302049</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>01</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-5514</ISSN><JournalIssue CitedMedium="Print"><Volume>108</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>09</Month></PubDate></JournalIssue><Title>Mycologia</Title><ISOAbbreviation>Mycologia</ISOAbbreviation></Journal><ArticleTitle>Nuclear DNA content of the hybrid plant pathogen Phytophthora andina determined by flow cytometry.</ArticleTitle><Pagination><MedlinePgn>899-904</MedlinePgn></Pagination><Abstract><AbstractText>Phytophthora andina is a heterothallic plant pathogen of Andean solanaceous hosts and is an interspecific hybrid of P. infestans and an unknown Phytophthora species. The objective of this study was to estimate the nuclear DNA content of isolates in three clonal lineages of P. andina relative to P. infestans Twelve isolates of P. andina and six isolates of P. infestans were measured for nuclear DNA content by propidium iodide-stained flow cytometry. We found that the DNA content of P. andina was similar but slightly smaller, on average, than that of our sample of P. infestans isolates. This is consistent with P. andina being a homoploid hybrid rather than allopolyploid hybrid. Nuclear DNA content was more variable among a smaller sample of P. infestans isolates, including a putative triploid isolate from Mexico, but small differences in nuclear DNA content were also observed among P. andina isolates. Both species appear to be able to tolerate significant variation in genome size.</AbstractText><CopyrightInformation>© 2016 by The Mycological Society of America.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jianan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, PO Box 110680, University of Florida, Gainesville, Florida 32611.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Presser</LastName><ForeName>Jackson W</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>School of Natural Resources and Environment and Emerging Pathogens Institute, PO Box 116455, University of Florida, Gainesville, Florida 32611.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goss</LastName><ForeName>Erica M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology and Emerging Pathogen Institute, PO Box 110680, University of Florida, Gainesville, Florida 32611 emgoss@ufl.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycologia</MedlineTA><NlmUniqueID>0400764</NlmUniqueID><ISSNLinking>0027-5514</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008800" MajorTopicYN="N">Mexico</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Phytophthora infestans</Keyword><Keyword MajorTopicYN="Y">flow cytometry</Keyword><Keyword MajorTopicYN="Y">interspecific hybrid</Keyword><Keyword MajorTopicYN="Y">ploidy</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27302049</ArticleId><ArticleId IdType="pii">15-107</ArticleId><ArticleId IdType="doi">10.3852/15-107</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Wang, Jianan" sort="Wang, Jianan" uniqKey="Wang J" first="Jianan" last="Wang">Jianan Wang</name></region><name sortKey="Goss, Erica M" sort="Goss, Erica M" uniqKey="Goss E" first="Erica M" last="Goss">Erica M. 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